周期素E2重組兔單克隆抗體

產品貨號：

BN42073R
中文名稱：

周期素E2重組兔單克隆抗體
英文名稱：

Rabbit anti-Cyclin E2 Monoclonal antibody
品牌：

Biorigin

貨號

產品規格

售價

備注
BN42073R-50ul

50ul

￥2020.00

交叉反應：Human(predicted:Mouse) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt
BN42073R-100ul

100ul

￥3240.00

交叉反應：Human(predicted:Mouse) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

產品描述

英文名稱	Cyclin E2
中文名稱	周期素E2重組兔單克隆抗體
別名	CCN E2; CCNE 2; CCNE2; CCNE2 protein; CYC E2; CYCE 2; CYCE2; CyclinE2; G1/S-specific cyclin E2; G1/S-specific cyclin E2; CCNE2_HUMAN.
研究領域	腫瘤細胞生物染色質和核信號信號轉導細胞周期蛋白
抗體來源	Rabbit
克隆類型	Monoclonal
克隆號	2B1
交叉反應	Human, (predicted: Mouse, )
產品應用	WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=2ug/Test ICC=1:50 IF=1:50-200 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	44kDa
細胞定位	細胞核
性狀	Liquid
濃度	1mg/ml
免疫原	Recombinant human Cyclin E2 protein, around C-terminal 100aa:
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產品介紹	The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells. Subunit: "Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Subcellular Location: Nucleus. Tissue Specificity: According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Post-translational modifications: Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway. Similarity: Belongs to the cyclin family. Cyclin E subfamily. SWISS: O96020 Gene ID: 9134 Database links: Entrez Gene: 9134 Human Entrez Gene: 12448 Mouse Entrez Gene: 362485 Rat Omim: 603775 Human SwissProt: O96020 Human SwissProt: Q7TMS8 Mouse SwissProt: Q9Z238 Mouse Unigene: 567387 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.