巢蛋白/神經上皮干細胞蛋白抗體

產品貨號：

BN41309R
中文名稱：

巢蛋白/神經上皮干細胞蛋白抗體
英文名稱：

Rabbit anti-Nestin Polyclonal antibody
品牌：

Biorigin

貨號

產品規格

售價

備注
BN41309R-100ul

100ul

￥2360.00

交叉反應：Human(predicted:Cow) 推薦應用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA
BN41309R-200ul

200ul

￥3490.00

交叉反應：Human(predicted:Cow) 推薦應用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

產品描述

英文名稱	Nestin
中文名稱	巢蛋白/神經上皮干細胞蛋白抗體
別名	ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
研究領域	細胞生物發育生物學神經生物學干細胞細胞類型標志物
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human, (predicted: Cow, )
產品應用	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	177kDa
細胞定位	細胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Nestin:21-120/1621
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產品介紹	Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Function: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Subunit: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3. Subcellular Location: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 Tissue Specificity: CNS stem cells. Post-translational modifications: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Similarity: Belongs to the intermediate filament family. SWISS: P48681 Gene ID: 10763 Database links: Entrez Gene: 10763 Human Entrez Gene: 18008 Mouse Entrez Gene: 25491 Rat Omim: 600915 Human SwissProt: P48681 Human SwissProt: Q6P5H2 Mouse SwissProt: P21263 Rat Unigene: 527971 Human Unigene: 331129 Mouse Unigene: 9701 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 神經干細胞標志物在動物體早期發育過程中，中樞神經系統發育尤為重要。Nestin是一種中等纖維蛋白，它在哺乳動物神經前體細胞中表達較高，目前已被廣泛用于神經前體細胞的重要標志分子。研究Nestin的表達調控規律，將有助于人們認識神經前體細胞的生物特性。此抗體還表達中樞神經系統干細胞及神經上皮細胞。