凋亡蛋白活性因子-1抗體（N端）

產品貨號：

BN40205R
中文名稱：

凋亡蛋白活性因子-1抗體（N端）
英文名稱：

Rabbit anti-APAF1(NT) Polyclonal antibody
品牌：

Biorigin

貨號

產品規格

售價

備注
BN40205R-100ul

100ul

￥2360.00

交叉反應：Human,Mouse,Rat(predicted:Chicken,Dog,Cow,Horse) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA
BN40205R-200ul

200ul

￥3490.00

交叉反應：Human,Mouse,Rat(predicted:Chicken,Dog,Cow,Horse) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

產品描述

英文名稱	APAF1(NT)
中文名稱	凋亡蛋白活性因子-1抗體（N端）
別名	APAF 1; APAF1; APAF-1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor; CED 4; CED4; KIAA0413; APAF_HUMAN; Apoptotic protease-activating factor 1.
研究領域	腫瘤細胞生物神經生物學信號轉導細胞凋亡
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, )
產品應用	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	137kDa
細胞定位	細胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Apaf-1:13-80/1248
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產品介紹	This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain(NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Subunit: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. Similarity: Contains 1 CARD domain. Contains 1 NB-ARC domain. Contains 13 WD repeats. SWISS: O14727 Gene ID: 317 Database links: Entrez Gene: 317 Human Entrez Gene: 11783 Mouse Entrez Gene: 78963 Rat Omim: 602233 Human SwissProt: O14727 Human SwissProt: O88879 Mouse SwissProt: Q9EPV5 Rat Unigene: 728891 Human Unigene: 220289 Mouse Unigene: 64522 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Apaf-1 （Apoptosis protease activating factor-1）調節細胞色素C依賴的Caspase-9原的自動催化活性，導致Caspase-3 的激活和引起凋亡。 Apaf-1在成年人的脾臟、外周血白細胞、腎臟、肺和胎兒腦、腎、肺中高水平表達。